Mrs Uju Kennedy-Ohnenye has withdrawn from the All Progressives Congress (APC) presidential race.
Nicholas announced his withdrawal while addressing delegates at APC Special National Convention on Tuesday in Abuja.
He directed his supporters to vote for Vice-President Yemi Osinbajo at the convention.
Former Gov. Godswill Akpabio and former Gov. Ibikunle Amosun had earlier withdrawn from the race urging his supporters to vote for Tinubu.